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Indigo PeptidesCertificate of analysis
Certificate
IP-COA-ND-261005
Issued
7 October 2026
Status
released

NAD+

Nicotinamide adenine dinucleotide, oxidised form

BatchIP-ND-261001This is the code printed on your vial.
Applicable strengths
  • 100 mg
  • 500 mg
  • 1000 mg
99.63%HPLC with UV detectionSpecification ≥ 99.0%
Full analytical results

Tests and specifications

TestMethodSpecificationResultStatus
AppearanceVisual inspectionWhite to off-white crystalline powderConformsPass
PurityHPLC with UV detection≥ 99.0%99.63%Pass
IdentityESI-MS[M+H]+ = 664.44 ± 0.50664.59Pass
Identity¹H NMRConforms to reference spectrumConformsPass
Related substancesHPLC, any single impurity≤ 0.5%0.17%Pass
Water contentKarl Fischer titration≤ 2.0%0.98%Pass
Residue on ignitionUSP <281>≤ 0.1%0.043%Pass
Heavy metalsICP-MS≤ 10 ppm< 1 ppmPass
Bacterial endotoxinsKinetic chromogenic LAL< 1.0 EU/mg< 0.125 EU/mgPass
SterilityMembrane filtration (USP <71>)No growthNo growthPass
Residual solventsHeadspace GC-FID (USP <467>)Acetonitrile ≤ 410 ppm, methanol ≤ 3000 ppm, dichloromethane ≤ 600 ppm (ICH Q3C)ConformsPass

Chromatogram

HPLC trace, NAD+, batch IP-ND-261001Reversed-phase HPLC trace. One dominant peak at 4.8 minutes, 99.63 percent of total peak area. Largest single impurity 0.17 percent.02505007501000051015202530mAURetention time (minutes)4.8 min99.63% area

HPLC trace for this batch. One dominant peak at 4.8 minutes, 99.63% of the total peak area, largest single impurity 0.17%.

Mass spectrum

ESI mass spectrum, NAD+, batch IP-ND-261001Electrospray ionisation mass spectrum. Base peak 664.59 m/z, charge state 1, expected 664.44.0255075100600650700750Relative intensity (%)m/z664.59[M+H]++Na

Electrospray ionisation. Expected 664.44, observed 664.59, a difference of 0.15 m/z at charge state 1. The smaller peak to its right is the sodium adduct, [M+Na]+, which electrospray produces alongside the protonated ion.

Reference data

Also known as
Nicotinamide adenine dinucleotide
CAS number
53-84-9
Molecular formula
C21H27N7O14P2
Molecular weight
663.43 g/mol
Modifications
The free acid, which is the basis an assay is specified against. Written as the +1 cation the formula gains a hydrogen and the mass is 664.44, so the two conventions differ by exactly one dalton.

Public reference values for the compound, not measurements on this batch. The batch measurements are in the table above.

Storage and handling

Unopened
2 to 8 °C, away from light and moisture
Long term
−20 °C, for the stated shelf life
Reconstituted
2 to 8 °C, use within 4 weeks
In transit
Stable at ambient temperature for up to 30 days
Reconstitute with
Bacteriostatic water

The vial this covers

The label on your vial carries the same batch code as this certificate, IP-ND-261001, and the QR goes to the results for it. If the code on the vial is not this one, the certificate for it is on this page under earlier batches.

Sticker shown at its printed size, 40 by 20 mm. Cap colour may vary by batch.

Release

Batch date
1 October 2026
Analysis date
4 October 2026
Issue date
7 October 2026
Best before
October 2028
Released by
Quality review, Indigo Peptides
Document
Valid only in its original, unmodified form

Results are taken from the batch testing documentation for this exact batch, supplied with the finished vials and held on file by us. Every value above is for the batch named on this certificate and for no other.

Holding an older vial?

BatchpurityAnalysed
IP-ND-26091299.57%15 September 2026

If the code on your vial is one of these, it is an earlier batch of ours and these are its results. A result we have published is never removed and never recalculated.

Take it with you

Download this certificate (PDF)

The PDF is this page on A4. Open the printable version to print it yourself.

IP-COA-ND-261005 · Batch IP-ND-261001indigopeptides.inResearch use only. Not for human or veterinary use.