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Indigo PeptidesCertificate of analysis
Certificate
IP-COA-GT-261007
Issued
6 October 2026
Status
released

Glutathione

Reduced L-glutathione, a tripeptide thiol

BatchIP-GT-261003This is the code printed on your vial.
Applicable strengths
  • 600 mg
99.52%HPLC with UV detectionSpecification ≥ 99.0%

Full analytical results

Tests and specifications

TestMethodSpecificationResultStatus
AppearanceVisual inspectionWhite to off-white crystalline powderConformsPass
PurityHPLC with UV detection≥ 99.0%99.52%Pass
IdentityESI-MS[M+H]+ = 308.33 ± 0.50308.40Pass
Identity¹H NMRConforms to reference spectrumConformsPass
Related substancesHPLC, any single impurity≤ 0.5%0.28%Pass
Water contentKarl Fischer titration≤ 2.0%0.78%Pass
Residue on ignitionUSP <281>≤ 0.1%0.012%Pass
Heavy metalsICP-MS≤ 10 ppm< 2 ppmPass
Bacterial endotoxinsKinetic chromogenic LAL< 1.0 EU/mg< 0.05 EU/mgPass
SterilityMembrane filtration (USP <71>)No growthNo growthPass
Residual solventsHeadspace GC-FID (USP <467>)Acetonitrile ≤ 410 ppm, methanol ≤ 3000 ppm, dichloromethane ≤ 600 ppm (ICH Q3C)ConformsPass

Chromatogram

HPLC trace, Glutathione, batch IP-GT-261003Reversed-phase HPLC trace. One dominant peak at 4.6 minutes, 99.52 percent of total peak area. Largest single impurity 0.28 percent.02505007501000051015202530mAURetention time (minutes)4.6 min99.52% area

HPLC trace for this batch. One dominant peak at 4.6 minutes, 99.52% of the total peak area, largest single impurity 0.28%.

Mass spectrum

ESI mass spectrum, Glutathione, batch IP-GT-261003Electrospray ionisation mass spectrum. Base peak 308.40 m/z, charge state 1, expected 308.33.0255075100200250300350400Relative intensity (%)m/z308.40[M+H]++Na

Electrospray ionisation. Expected 308.33, observed 308.40, a difference of 0.07 m/z at charge state 1. The smaller peak to its right is the sodium adduct, [M+Na]+, which electrospray produces alongside the protonated ion.

Reference data

Also known as
GSH
CAS number
70-18-8
Molecular formula
C10H17N3O6S
Molecular weight
307.32 g/mol
Sequence
gamma-Glu-Cys-Gly
Modifications
The glutamate links through its gamma carboxyl rather than the alpha, so a one-letter code would misdescribe the molecule. The cysteine thiol is free, which is the reduced form; the single sulfur in the formula is itself the proof it is not the oxidised dimer.

Public reference values for the compound, not measurements on this batch. The batch measurements are in the table above.

Storage and handling

Unopened
2 to 8 °C, away from light and moisture
Long term
−20 °C, for the stated shelf life
Reconstituted
2 to 8 °C, use within 4 weeks
In transit
Stable at ambient temperature for up to 30 days
Reconstitute with
Bacteriostatic water

The vial this covers

The label on your vial carries the same batch code as this certificate, IP-GT-261003, and the QR goes to the results for it. If the code on the vial is not this one, the certificate for it is on this page under earlier batches.

Sticker shown at its printed size, 40 by 20 mm. Cap colour may vary by batch.

Release

Batch date
3 October 2026
Analysis date
5 October 2026
Issue date
6 October 2026
Best before
October 2028
Released by
Quality review, Indigo Peptides
Document
Valid only in its original, unmodified form

Results are taken from the batch testing documentation for this exact batch, supplied with the finished vials and held on file by us. Every value above is for the batch named on this certificate and for no other.

Holding an older vial?

BatchpurityAnalysed
IP-GT-26091299.31%14 September 2026

If the code on your vial is one of these, it is an earlier batch of ours and these are its results. A result we have published is never removed and never recalculated.